Camellia nitidissima Chi Extract Potentiates the Sensitivity of Gastric Cancer Cells to Paclitaxel via the Induction of Autophagy and Apoptosis
Identifieur interne : 000B67 ( Main/Exploration ); précédent : 000B66; suivant : 000B68Camellia nitidissima Chi Extract Potentiates the Sensitivity of Gastric Cancer Cells to Paclitaxel via the Induction of Autophagy and Apoptosis
Auteurs : Xu He [République populaire de Chine] ; Hang Li [République populaire de Chine] ; Meixiao Zhan [République populaire de Chine] ; Hongyang Li [République populaire de Chine] ; Aiqun Jia [République populaire de Chine] ; Sensen Lin [République populaire de Chine] ; Li Sun [République populaire de Chine] ; Hongzhi Du [République populaire de Chine] ; Shengtao Yuan [République populaire de Chine] ; Yong Li [République populaire de Chine]Source :
- OncoTargets and therapy [ 1178-6930 ] ; 2019.
Abstract
Camellia nitidissima Chi (CNC) has been applied as a traditional folk medicine for the effective treatment of various diseases. However, there is little research regarding the mechanism of CNC on pharmaceutical function including anticancer effect.
JHC-4 is a n-butanol extract from CNC. The anti-proliferation effect was evaluated by MTT assays. Monodansylcadaverine (MDC) staining, Western blotting and autophagy inhibitors (CQ and BafA1) were applied to determine whether JHC-4 induced autophagy. The synergistic anticancer effect was evaluated by MTT assays, flow cytometry, Western blotting and autophagy inhibitors. Western blotting was used to explore the influence of PI3K/Akt/mTOR signaling pathway induced by drug treatment.
JHC-4 caused significant growth inhibition and induced autophagy in human gastric cancer cells. Moreover, JHC-4 as an autophagy agonist synergistically potentiated the sensitivity of gastric cancer cells to paclitaxel. Meanwhile, JHC-4 could significantly enhance the growth inhibition effect of paclitaxel by the induction of autophagy and apoptosis. Finally, we demonstrated that the PI3K/Akt/mTOR signaling pathway was involved in the synergistic anti-proliferation effect of JHC-4 and paclitaxel.
All these data indicated that JHC-4 was a novel autophagy inducer when combination with paclitaxel for gastric cancer, which provided the scientific evidence for the use of this Chinese traditional medicine against cancer.
Url:
DOI: 10.2147/OTT.S220453
PubMed: 31853183
PubMed Central: 6914663
Affiliations:
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China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Sensen" sort="Lin, Sensen" uniqKey="Lin S" first="Sensen" last="Lin">Sensen Lin</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Li" sort="Sun, Li" uniqKey="Sun L" first="Li" last="Sun">Li Sun</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for 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Yuan</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Yong" sort="Li, Yong" uniqKey="Li Y" first="Yong" last="Li">Yong Li</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Zhuhai Interventional Medical Center, Zhuhai Precision Medical Center, Zhuhai People’s Hospital, Zhuhai Hospital Affiliated with Jinan University</institution>,<addr-line>Zhuhai</addr-line><addr-line>519000</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de 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strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Hang" sort="Li, Hang" uniqKey="Li H" first="Hang" last="Li">Hang Li</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhan, Meixiao" sort="Zhan, Meixiao" uniqKey="Zhan M" first="Meixiao" last="Zhan">Meixiao Zhan</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Zhuhai Interventional Medical Center, Zhuhai Precision Medical Center, Zhuhai People’s Hospital, Zhuhai Hospital Affiliated with Jinan University</institution>,<addr-line>Zhuhai</addr-line><addr-line>519000</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Hongyang" sort="Li, Hongyang" uniqKey="Li H" first="Hongyang" last="Li">Hongyang Li</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College</institution>,<addr-line>Nanjing</addr-line><addr-line>210042</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Jia, Aiqun" sort="Jia, Aiqun" uniqKey="Jia A" first="Aiqun" last="Jia">Aiqun Jia</name><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>School of Environmental and Biological Engineering, Nanjing University of Science and Technology</institution>,<addr-line>Nanjing</addr-line><addr-line>210094</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Sensen" sort="Lin, Sensen" uniqKey="Lin S" first="Sensen" last="Lin">Sensen Lin</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Li" sort="Sun, Li" uniqKey="Sun L" first="Li" last="Sun">Li Sun</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Du, Hongzhi" sort="Du, Hongzhi" uniqKey="Du H" first="Hongzhi" last="Du">Hongzhi Du</name><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>School of Pharmacy, Hubei University of Chinese Medicine</institution>,<addr-line>Wuhan</addr-line><addr-line>430065</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, Shengtao" sort="Yuan, Shengtao" uniqKey="Yuan S" first="Shengtao" last="Yuan">Shengtao Yuan</name><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University</institution>,<addr-line>Nanjing</addr-line><addr-line>210009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Yong" sort="Li, Yong" uniqKey="Li Y" first="Yong" last="Li">Yong Li</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Zhuhai Interventional Medical Center, Zhuhai Precision Medical Center, Zhuhai People’s Hospital, Zhuhai Hospital Affiliated with Jinan University</institution>,<addr-line>Zhuhai</addr-line><addr-line>519000</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">OncoTargets and therapy</title><idno type="eISSN">1178-6930</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S2001"><title>Background</title><p>Camellia nitidissima Chi (CNC) has been applied as a traditional folk medicine for the effective treatment of various diseases. However, there is little research regarding the mechanism of CNC on pharmaceutical function including anticancer effect.</p></sec><sec id="S2002"><title>Materials and methods</title><p>JHC-4 is a n-butanol extract from CNC. The anti-proliferation effect was evaluated by MTT assays. Monodansylcadaverine (MDC) staining, Western blotting and autophagy inhibitors (CQ and BafA1) were applied to determine whether JHC-4 induced autophagy. The synergistic anticancer effect was evaluated by MTT assays, flow cytometry, Western blotting and autophagy inhibitors. Western blotting was used to explore the influence of PI3K/Akt/mTOR signaling pathway induced by drug treatment.</p></sec><sec id="S2003"><title>Results</title><p>JHC-4 caused significant growth inhibition and induced autophagy in human gastric cancer cells. Moreover, JHC-4 as an autophagy agonist synergistically potentiated the sensitivity of gastric cancer cells to paclitaxel. Meanwhile, JHC-4 could significantly enhance the growth inhibition effect of paclitaxel by the induction of autophagy and apoptosis. Finally, we demonstrated that the PI3K/Akt/mTOR signaling pathway was involved in the synergistic anti-proliferation effect of JHC-4 and paclitaxel.</p></sec><sec id="S2004"><title>Conclusion</title><p>All these data indicated that JHC-4 was a novel autophagy inducer when combination with paclitaxel for gastric cancer, which provided the scientific evidence for the use of this Chinese traditional medicine against cancer.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="He, Xu" sort="He, Xu" uniqKey="He X" first="Xu" last="He">Xu He</name></noRegion><name sortKey="Du, Hongzhi" sort="Du, Hongzhi" uniqKey="Du H" first="Hongzhi" last="Du">Hongzhi Du</name><name sortKey="Jia, Aiqun" sort="Jia, Aiqun" uniqKey="Jia A" first="Aiqun" last="Jia">Aiqun Jia</name><name sortKey="Li, Hang" sort="Li, Hang" uniqKey="Li H" first="Hang" last="Li">Hang Li</name><name sortKey="Li, Hongyang" sort="Li, Hongyang" uniqKey="Li H" first="Hongyang" last="Li">Hongyang Li</name><name sortKey="Li, Yong" sort="Li, Yong" uniqKey="Li Y" first="Yong" last="Li">Yong Li</name><name sortKey="Lin, Sensen" sort="Lin, Sensen" uniqKey="Lin S" first="Sensen" last="Lin">Sensen Lin</name><name sortKey="Sun, Li" sort="Sun, Li" uniqKey="Sun L" first="Li" last="Sun">Li Sun</name><name sortKey="Yuan, Shengtao" sort="Yuan, Shengtao" uniqKey="Yuan S" first="Shengtao" last="Yuan">Shengtao Yuan</name><name sortKey="Zhan, Meixiao" sort="Zhan, Meixiao" uniqKey="Zhan M" first="Meixiao" last="Zhan">Meixiao Zhan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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